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Mimic of green fluorescent protein (GFP) fluorophore

(5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl) -4H-imidazol-4-one

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RNT-002-1 1 mg 130,00 Add to Basket/Quote Add to Notepad
Structural formula of DFHBI-1T (Mimic of green fluorescent protein (GFP) fluorophore, (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl) -4H-imidazol-4-one)
Structural formula of DFHBI-1T

For in vitro use only!

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months after date of delivery

Molecular Formula: C13H9F5N2O2

Molecular Weight: 320.22 g/mol

Exact Mass: 320.06 g/mol

CAS#: 1539318-36-9

Purity: ≥ 95 % (HPLC)

Form: solid

Color: yellow to orange

Solubility: DMSO

DFHBI-1T is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-1T-specific RNA sequence (DFHBI-1T aptamer) such as Spinach2 or Broccoli.[1-3] DFHBI-1T possesses a higher photostability than DFHBI which generally results in a higher signal intensity and lower background especially in combination with Broccoli.

Fluorescence is activated upon binding to a matching aptamer sequence:

Spinach2/DFHBI-1T complex[1]:,
Exc.max: 482 nm / Em.max: 505 nm / ɛmax: 31000 M-1 cm-1

Broccoli/DFHBI complex[2]:
Exc.max: 472 nm / Em.max: 507 nm / ɛmax: 29600 M-1 cm-1

Selected References:
[1] Song et al. (2014) Plug-and-Play fluorophores extend the Spectral Properties of Spinach. J. Am. Chem. Soc. 136:1198.
[2] Filonov et al. (2014) Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution. J. Am. Chem. Soc. 136:16299.
[3] Filonov et al. (2015) In-gel imaging of RNA processing using broccoli reveals optimal aptamer expression strategies. Chem. Biol. 22(5):649.