Mimic of green fluorescent protein (GFP) fluorophore
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H10F2N2O2
Molecular Weight: 252.22 g/mol
Purity: ≥ 98 % (HPLC)
Form: yellow solid
DFHBI is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHBI-specific RNA sequence (DFHBI aptamer) such as Spinach, Spinach2 or Broccoli.[1-3]
Fluorescence is activated upon binding to a matching aptamer sequence:
Exc.max: 469 nm / Em.max: 501 nm / ɛmax: 24271 M-1 cm-1
Exc.max: 447 nm / Em.max: 501 nm / ɛmax: 22000 M-1 cm-1
 Paige et al. (2011) RNA mimics of green fluorescent protein. Science 333(6042):642.
 Song et al. (2014) Plug-and-Play fluorophores extend the Spectral Properties of Spinach. J. Am. Chem. Soc. 136:1198.
 Filonov et al. (2014) Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution. J. Am. Chem. Soc. 136:16299.