WNV Envelope
recombinant, E. coli
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PR-1275 | 100 μg | 300,70 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 20 mM Phosphate buffer pH 7.5)
Applications:
Antigen in ELISA and Western Blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
Description:
The E. coli derived recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused to 6xHis-tag. Mw ~42 kDa.
West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus, both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDa protein blocks, the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
The protein is purified by proprietary chromatographic technique.
BIOZ Product Citations:
Selected References:
Saxena et al. (2013) Cloning and expression of an envelope gene of West Nile virus and evaluation of the protein for use in an IgM ELISA.Diagn Microbiol Infect Dis. 75:396.
Vogt et al. (2011) Poorly neutralizing cross-reactive antibodies against the fusion loop of West Nile virus envelope protein protect in vivo via Fcgamma receptor and complement-dependent effector mechanisms.J Virol. 85:11567.
Wang et al. (2001) West Nile virus envelope protein: role in diagnosis and immunity.Ann N Y Acad Sci. 325:325.
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