Toxoplasma Gondii Surface Antigen 30
Toxoplasma gondii, recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 47 kDa ( presented by a major band and minor smaller size bands)
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 60 mM NaCl, 10 mM glutathione, 0.25% sarcosyl and 50% glycerol)
Antigen in ELISA and Western blots, excellent antigen for detection of Toxoplasma gondii with minimal specificity problems.
Toxoplasma gondii p30 is tagged with GST, His and S at the N-terminus. The protein contains the p30 (SAG1) immunodominant regions, amino acids 45-198. The protein is purified by proprietary chromatographic technique.
Background: Toxoplasma gondii is an obligate intracellular protozoan parasite that infects all warmblooded animals, including humans, and causes toxoplasmosis. SAG1, the major surface molecule of Toxoplasma gondii, is an important attachment ligand for the host cell. It is a highly immunogenic protein which has generated great interest as a diagnostic reagent, as a potential subunit vaccine, and for its role in invasion.
Specificity: Immunoreactive with sera of T. gondii- infected individuals.
Hiszczynska-Sawicka et al. (2003) High yield expression and singlestep purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expr. Purif. 27:150.
Robinson et al. (2004) Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding. Parasitology. 128:391.
Seng et al. (2004) SAG1 is a host-targeted antigen for protection against toxoplasma gondii infection. Pathobiology. 71:144.
Chen et al. (2003) Induction of immune responses in mice by vaccination with Liposome-entrapped DNA complexes encoding Toxoplasma gondii SAG1 and ROP1 genes. Chin. Med. J. (Engl) 116:1561.
Sager et al. (2003) Immunodiagnosis of primary Toxoplasma gondii infection in sheep by the use of a P30 IgG avidity ELISA. Parasitol. Res. 91:171.
Mohamed et al. (2003) Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes. Vaccine. 21:2852.