TG GRA7/p29 (residues 24-100)

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 1.5 M urea and 50% glycerol)

Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of Toxoplasma gondii with minimal specificity problems.

Description:
The protein contains the p29 (GRA7) immunodominant regions, amino acids 24-100. The protein is purified by proprietary chromatographic technique.

Background: Toxoplasma gondii is an obligate intracellular protozoan parasite that infects all warmblooded animals, including humans, and causes toxoplasmosis. GRA7 is a recently discovered 29-kDa protein. Like GRA1, it is secreted from the dense granules, and it reacts with sera from humans with acute and chronic infections.

Specificity: Immunoreactive with sera of T. gondii- infected individuals.

BIOZ Product Citations:

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Selected References:
Hiszczynska-Sawicka et al. (2003) High yield expression and singlestep purification of Toxoplasma gondii SAG1, GRA1, and GRA7 antigens in Escherichia coli. Protein Expr. Purif. 27:150.
Neudeck et al. (2002) Expression variance, biochemical and immunological properties of Toxoplasma gondii dense granule protein GRA7. Microbes Infect. 4:581.
Vercammen et al. (2000) DNA vaccination with genes encoding Toxoplasma gondii antigens GRA1, GRA7, and ROP2 induces partially protective immunity against lethal challenge in mice. Infect. Immun. 68:38.
Ferguson et al. (1999) In vivo expression and distribution of dense granule protein 7 (GRA7) in the exoenteric (tachyzoite, bradyzoite) and enteric (coccidian) forms of Toxoplasma gondii. Parasitology. 119:259.
Jacobs et al. (1999) Evaluation of recombinant dense granule antigen 7 (GRA7) of Toxoplasma gondii for detection of immunoglobulin G antibodies and analysis of a major antigenic domain. Clin. Diagn. Lab. Immunol. 6:24.
Bonhomme et al. (1998) Quantitative immunolocalization of a P29 protein (GRA7), a new antigen of toxoplasma gondii. J. Histochem. Cytochem. 46:1411.