SARS-Associated Coronavirus Nucleocapsid
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 32 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl, 60 mM NaCl and 50% glycerol)
SARS-ACN contains Nucleocapsid protein immunodominant regions, amino acids: 1-49, 192-220. SARS-ACN is purified by proprietary chromatographic techniques.
Background: SARS (Severe Acute Respiratory Syndrome) Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Recombinant SARS-ACN Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.
Specificity: Immunoreactive with sera of SARSinfected individuals.
Liu et al. (2004) High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris. World J. Gastroenterol. 10:3602.
Luo et al. (2004) Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A. Biochem. Biophys. Res. Commun. 321:557.
Wang et al. (2004) Low stability of nucleocapsid protein in SARS virus. Biochemistry 43:11103.
Lau et al. (2004) Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 42:2884.
Woo et al. (2004) Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus. Clin. Diagn. Lab. Immunol. 11:665.
Leung et al. (2004) Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis. 190:379.