Human Immunodeficiency Virus 2 Antigen, coat glycoprotein
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 20 mM PBS pH 7.8, 0.5 M NaCl, 1 mM DTT, 8 M urea and 0.4 M imidazole)
The protein contains the a fragment of the HIV-2 coat protein gp36, amino acids: (390-702), molecular weight 34 kDa. The protein was purified by proprietary chromatographic technique.
Background: HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. Like all retroviruses, HIV-2 encodes gag (nucleocapsid), pol (polymerase), and env (envelope) genes. The env gene, which demonstrates considerable variation from isolate to isolate encodes the gp160/140 precursor of the gp120 outer membrane glycoprotein and the gp32-40 transmembrane glycoproteins.
May be used in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
Specificity: Immuno reactive with all sera of HIV-I infected individuals.
Chen et al. (2000) HIV-2 transmembrane protein gp36 binds to the putative cellular receptor proteins P45 and P62. Immunobiology. 201:317.
Chen et al. (1995) HIV-2 transmembrane protein gp36 like HIV-1 gp41 binds to human lymphocytes and monocytes. AIDS. 9:1193.
Imberti et al. (1995) Insertion of a short human immunodeficiency virus (HIV)-2 gp36 sequence into an HIV-1 p24 recombinant protein results in a polypeptide with potent and TCRBV-restricted T cell triggering activity. Eur. J. Immunol. 25:218.