Human Immunodeficiency Virus 2 Antigen, coat glycoprotein
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 20 mM PBS pH 7.8, 0.5 M NaCl, 1 mM DTT, 8 M urea and 0.4 M imidazole)
May be used in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
The protein contains the full-length sequence of HIV-2 coat protein gp32. The protein was purified by proprietary chromatographic technique.
Background: HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. Like all retroviruses, HIV-2 encodes gag (nucleocapsid), pol (polymerase), and env (envelope) genes. The env gene, which demonstrates considerable variation from isolate to isolate encodes the gp160/140 precursor of the gp120 outer membrane glycoprotein and the gp32-40 transmembrane glycoprotein.
Specificity: Immunoreactive with all sera of HIV-2 infected individuals.
Markovitz et al. (1993) Infection with the Human Immunodeficiency Virus Type 2. Ann. Int. Med. 118:211.
Norrby et al. (1989) Site-directed enzyme-linked immunosorbent assay with a synthetic simian immunodeficiency virus SIVmac peptide identifying antibodies against the HIV-2 transmembrane glycoprotein. AIDS. 3:17.