Human Immunodeficiency Virus-1, Negative Factor
recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 5 mM Tris-HCl pH 8.0)
Recombinant N-terminal His-tagged full-length HIV-1 Nef protein (SF2 strain). Human immunodeficiency virus type 1 (HIV-1) Nef is a membrane-associated protein that is produced at the earliest stage of viral gene expression and is a component of viral particles. Nef has been demonstrated to bind and downregulate cell-surface receptors CD4 and MHC I. In addition, Nef has been reported to have diverse effects on cellular signal transduction pathways. It interacts with various cellular protein kinases and acts both as a kinase substrate and as a modulator of kinase activity.
Cullen et al. (1994) The role of Nef in the replication cycle of the human and simian immunodeficiency viruses. Virology 205:1.
Grzesiek et al. (1996) The solution structure of HIV-1 Nef reveals an unexpected fold and permits delineation of the binding surface for the SH3 domain of Hck tyrosine protein kinase. Nat. Struct. Biol. 3:340.
Preusser et al. (2001) Direct in vitro binding of full-length HIV-1 Nef protein to CD4 cytoplasmic domain. J. Virol. 75:3960.