Human Immunodeficiency Virus 1 Antigen
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 55 kDa
Purity: > 90 % (SDS-PAGE, HPLC)
Form: liquid (Supplied in 10 mM Tris-HCl pH 8.0, 140 mM NaCl and 400 mM L-Arginine. For long time storage add a carrier protein like 0.1% BSA)
Antigen in ELISA and Western blots, excellent antigen for detection of HIV seroconvertors with minimal specificity problems.
Recombinant HIV-1 p55 gag is glycosylated with N-linked sugars and produced in insect cells. Recombinant HIV-1 p55 is purified by proprietary chromatographic technique.
Background: HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. p55 is a precursor protein, which is processed to p17 (matrix), p24 (capsid), p7 (nucleocapsid), and p6 proteins by the viral protease. It is called 'assemblin' to indicate its role in viral assembly.
Specificity: Immunoreactive with sera of HIV-infected individuals.
Ivanov et al. (2001) Sup35p yeast prion-like protein as an adapter for production of the Gag-p55 antigen of HIV-1 and the L-chain of botulinum neurotoxin in Saccharomyces cerevisiae. Res. Microbiol. 152:27.
Neidleman et al. (2000) Genetically detoxified mutants of heat-labile enterotoxin from Escherichia coli are effective adjuvants for induction of cytotoxic T-cell responses against HIV-1 gag-p55. Immunology 101:154.
Kazzaz et al. (2000) Novel anionic microparticles are a potent adjuvant for the induction of cytotoxic T lymphocytes against recombinant p55 gag from HIV-1. J. Control. Release 67:347.
Bristow et al. (1999) Human cyclophilin has a significantly higher affinity for HIV-1 recombinant p55 than p24. J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 20:334.
Jakobsen et al. (1998) Increased levels of soluble tumour necrosis factor receptor-I (P55) and decreased IgG1 reactivities in HIV-1 patients with cytomegalovirus disease. Scand. J. Immunol. 47:591.
McAdam et al. (1998) Cross-clade recognition of p55 by cytotoxic T lymphocytes in HIV-1 infection. AIDS 12:571.