Herpes Simplex Virus-1 glycoprotein G
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM Tris-HCl, 1 mM EDTA and 50% glycerol)
Recombinant HSV-1 gG contains the immunodominant regions of glycoprotein G, amino acids: 84-175. HSV-1-gG is purified by proprietary chromatographic techniques.
Background: Glycoprotein G-1 (gG-1), a viral envelope glycoprotein that was suggested to contribute to viral entry through apical surfaces of polarized cells, has been utilized as a prototype antigen for HSV-1 type-specific diagnosis due to lack of cross-reactivity with its counterpart in HSV-2, i.e., gG-2. Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response.
Recombinant rHSV-1 gG Antigen may be used in ELISA and Western blots, excellent for detection of HSV with minimal specificity problems.
Specificity: Immunoreactive with sera of HSV-infected individuals.
Rekabdar et al. (2002) Dichotomy of glycoprotein g gene in herpes simplex virus type 1 isolates. J. Clin. Microbiol. 40:3245.
Tunback et al. (2000) Glycoprotein G of herpes simplex virus type 1: identification of type-specific epitopes by human antibodies. J. Gen. Virol. 81:1033.
Anderson et al. (2000) Pseudotyping of glycoprotein D-deficient herpes simplex virus type 1 with vesicular stomatitis virus glycoprotein G enables mutant virus attachment and entry. J. Virol. 74:2481.
Rekabdar et al. (1999) Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1. Clin. Diagn. Lab. Immunol. 6:826.
Sullivan et al. (1987) Expression and characterization of herpes simplex virus type 1 (HSV-1) glycoprotein G (gG) by recombinant vaccinia virus: neutralization of HSV-1 infectivity with anti-gG antibody. J. Gen. Virol. 68:2587.