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HEV-ORF2 (residues 403-461)

Hepatitis E Virus Open Reading Frame protein

recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
HEV-ORF2 (residues 403-461) PR-1186 100 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 8 M urea and 10 mM beta-mercaptoethanol)

pH: 8.0

Description:
The protein is fused with beta-galactosidase at the N-terminus and contains immunodominant HEV ORF2 fragment, amino acids: 403-461. Hepatitis E Virus protein is purified by proprietary chromatographic techniques.

Background: Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.

Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.

Specificity: Immunoreactive with sera of HEV-infected individuals.

Selected References:
Surjit et al. (2004) The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J. Virol. 78:320.
Tyagi et al. (2001) The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize. Biochem. Biophys. Res. Commun. 286:214.
Tuteja et al. (2000) Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes. Viral. Immunol. 13:169.
Li et al. (2000) Recombinant subunit ORF2.1 antigen and induction of antibody against immunodominant epitopes in the hepatitis E virus capsid protein. J. Med. Virol. 60:379.