Hepatitis E Virus Open Reading Frame proteins, 4 immunodominant regions
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl, 1 mM EDTA, 0.5 M urea and 50% glycerol)
Antigen in ELISA and Western blots, excellent antigen for detection of HEV with minimal specificity problems.
The protein is a mosiac of 4 immunodominant HEV ORF2-ORF3 fragments. Hepatitis E Virus protein is purified by proprietary chromatographic techniques.
Background: Hepatitis E virus (HEV) is a major human pathogen in much of the developing world. It is a plus-strand RNA virus with a 7.2-kb polyadenylated genome consisting of three open reading frames, ORF1, ORF2, and ORF3. Of these, ORF2 encodes the major capsid protein of the virus and ORF3 encodes a small protein of unknown function.
Specificity: Immunoreactive with sera of HEV-infected individuals.
Surjit et al. (2004) The ORF2 protein of hepatitis E virus binds the 5' region of viral RNA. J. Virol. 78:320.
Tyagi et al. (2001) The full-length and N-terminal deletion of ORF2 protein of hepatitis E virus can dimerize. Biochem. Biophys. Res. Commun. 286:214.
Tuteja et al. (2000) Augmentation of immune responses to hepatitis E virus ORF2 DNA vaccination by codelivery of cytokine genes. Viral. Immunol. 13:169.
Li et al. (2000) Recombinant subunit ORF2.1 antigen and induction of antibody against immunodominant epitopes in the hepatitis E virus capsid protein. J. Med. Virol. 60:379.
Tyagi et al. (2004) The ORF3 protein of hepatitis E virus interacts with liver-specific alpha1-microglobulin and its precursor alpha1-microglobulin/bikunin precursor (AMBP) and expedites their export from the hepatocyte. J. Biol. Chem. 279:29308.
Tyagi et al. (2001) Self-association and mapping of the interaction domain of hepatitis E virus ORF3 protein. J. Virol. 75:2493.
Zafrullah et al. (1997) The ORF3 protein of hepatitis E virus is a phosphoprotein that associates with the cytoskeleton. J. Virol. 71:9045.
Lal et al. (1997) Expression and characterization of the hepatitis E virus ORF3 protein in the methylotrophic yeast, Pichia pastoris. Gene. 190:63.