Hepatitis C Virus Non-Structural protein, Genotype 3
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 7.5, 1 mM EDTA, 1.5 M urea and 50% glycerol)
Recombinant HCV-NS4 contains the fragments of the NS4 immunodominant regions: amino acids 1691-1710, 1712-1733, and 1921-1940. Hepatitis C Virus NS4 is purified by proprietary chromatographic techniques.
Background: The genome of hepatitis C virus (HCV) consists of seven functional regions: the core, E1, E2/NS1, NS2, NS3, NS4, and NS5 regions. Since the discovery of HCV, significant progress in the development of serologic tests for the detection of antibodies to HCV has been made. The earliest tests, developed for blood screening, were enzyme immunoassays (EIAs) that detect antibody to a cloned HCV NS4 protein (C100). The commercial EIAs that have been developed to date have used synthetic peptides or recombinant chimeric polyproteins as antigens.
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Specificity: Immunoreactive with sera of HCV-infected individuals.
Chu et al. (2004) Low-level hepatitis C viremia and humoral immune response to NS4 in chronic hepatitis B virus-hepatitis C virus coinfection. Scand. J. Gastroenterol. 39:778.
Masalova et al. (2002) Characterization of monoclonal antibodies and epitope mapping of the NS4 protein of hepatitis C virus. Immunol. Lett. 83:187.
Okamoto et al. (2000) Prospective reevaluation of risk factors in mother-to-child transmission of hepatitis C virus: high virus load, vaginal delivery, and negative anti-NS4 antibody. J. Infect. Dis. 182:1511.
Chien et al. (1999) Use of a Novel Hepatitis C Virus (HCV) Major-Epitope Chimeric Polypeptide for Diagnosis of HCV Infection. J. Clin. Microbiol. 37:1393.