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HCV-NS3-S1b (residues 1192-1459)

Hepatitis C Virus Non-Structural protein, Subtype 1b

recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-1147-1 1 mg 977,50 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 5 mM glutathione, 1.5 M urea, 0.2% Triton X-100 and 50% glycerol)

pH: 8.0

The protein contains the full-length HCV NS3 (c33c) immunodominant region, amino acids 1192-1459.The protein is fused to a GST-tag at the N-Terminus. Hepatitis C NS3 protein is purified by proprietary chromatographic techniques.

Background: The nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region. Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around amino acids 1250/1251), A (within amino acids 1250-1334), A/B (around amino acids 1323 and 1334), and B/C (around amino acids 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis.

Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Specificity: Immunoreactive with sera of HCV-infected individuals.

Selected References:
Gal-Tanamy et al. (2005) HCV NS3 serine protease-neutralizing single-chain antibodies isolated by a novel genetic screen. J. Mol. Biol. 347:991.
Duan et al. (2004) Antiviral compounds from traditional Chinese medicines Galla Chinese as inhibitors of HCV NS3 protease. Bioorg. Med. Chem. Lett. 14:6041.
Sun et al. (2004) P4 cap modified tetrapeptidyl alpha-ketoamides as potent HCV NS3 protease inhibitors. Bioorg. Med. Chem. Lett. 14:4333.
Nizi et al. (2004) Capped dipeptide phenethylamide inhibitors of the HCV NS3 protease. Bioorg. Med. Chem. Lett. 14:2151.
Liu et al. (2003) Double-stranded DNA-induced localized unfolding of HCV NS3 helicase subdomain 2. Protein Sci. 12:2757.
Hedge et al. (2003) Two antiviral compounds from the plant Stylogne cauliflora as inhibitors of HCV NS3 protease. Bioorg. Med. Chem. Lett. 13:2925.