Hepatitis C Virus Nucleocapsid
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 136 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 20 mM Tris-HCl pH 8.0, 8 M urea and 10 mM beta-mercaptoethanol)
The protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-192, with a molecular weight of 22 kDa. The protein is fused with b-galactosidase (114 kDa) at the N-terminus. Hepatitis C Virus core proteins are purified by proprietary chromatographic techniques.
Background: The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER) Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition, HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
Specificity: Immunoreactive with sera of HCV-infected individuals.
Kang et al. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics 5:2227.
Fukutomi et al. (2005) Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression. Hepatology 41:1096.
Gaudy et al. (2005) Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup. J. Clin. Microbiol. 43:1722.
Lindh et al. (2005) Monitoring treatment response by the hepatitis C virus core antigen assay. Eur. J. Clin. Microbiol. Infect. Dis. 24:230.
Boni et al. (2005) Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280:17737.