» Sign in

HCV-N24

Hepatitis C Virus Nucleocapsid

recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-1142-1 1 mg 977,50 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 20 mM Tris-HCl pH 8, 1.5 M urea and 10 mM beta-mercaptoethanol)

pH: 8.0

Description:
The protein contains the HCV core nucleocapsid immunodominant regions. The protein is fused with b-galactosidase (114 kDa) at the N-terminus. Hepatitis C Virus core proteins are purified by proprietary chromatographic techniques.

Background: The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum (ER) Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However, the mechanisms of core-mediated transcriptional regulation remain poorly understood. Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways In addition, HCV core antigen is a recently developed marker of hepatitis C infection. It is remarkably efficient at establishing persistent infection, suggesting that it has evolved one or more strategies aimed at evading the host immune response. T cell responses, including interferon-gamma production, are severely suppressed in chronic HCV patients. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.

Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Specificity: Immunoreactive with sera of HCV-infected individuals.

Selected References:
Kang et al. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics 5:2227.
Fukutomi et al. (2005) Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression. Hepatology 41:1096.
Gaudy et al. (2005) Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup. J. Clin. Microbiol. 43:1722.
Lindh et al. (2005) Monitoring treatment response by the hepatitis C virus core antigen assay. Eur. J. Clin. Microbiol. Infect. Dis. 24:230.
Boni et al. (2005) Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280:17737.