Hepatitis B Virus Surface Antigen, adw subtype
recombinant, Pichia pastoris
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at 4 °C
Shelf Life: 12 months
Molecular Weight: 24 kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 20 mM phosphate buffer pH 7.2 and 154 mM NaCl)
The protein contains the Hepatitis B Virus Surface Antigen immunodominant region and is purified by proprietary chromatographic techniques.
Background: Hepatitis B virus (HBV) is a small enveloped virus that belongs to the hepadnavirus family. The genome of the hepatitis B virus (HBV), a partially doublestranded circular DNA, has four known genes encoding the viral surface proteins (pre-S 1, pre-S2 and HBsAg), the precore (pre-C) and core (C) proteins (HBeAg and HBcAg), the DNA polymerase, the X protein. There are distinct subtypes of HBV indicative of strain heterogeneity. The subtypes are distinguished by antigenic determinants on the surface antigen (HBsAg) and their corresponding antibodies. There is a common group determinant, a, which appears in all HBsAg specimens. There are two sets of subdeterminants, d or y and w or r, which appear to be allelic or mutually exclusive and which are used for the identification of subtypes. Thus, there are at least four major groups into which HBsAg can be classified: adw, adr, ayw, and ayr.
Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Specificity: Immunoreactive with sera of HBV-infected individuals.
Wai-Kuo Shih et al. (1991) Strain Analysis of Hepatits B Virus on the Basis of Restriction Endonuclease Analysis of Polymerase Chain Reaction Products. J. Clin. Microbiol. 29:1640.
Yang et al. (2005) Gene cloning, bacterial expression, in vitro refolding, and characterization of a single-chain Fv antibody against PreS1 (21-47) fragment of HBsAg. Protein Expr. Purif. 41:341.
Li et al. (2005) Oligodeoxynucleotides containing synthetic immunostimulatory motifs augment potent Th1 immune responses to HBsAg in mice. Int. Immunopharmacol. 5:981.