Hepatitis B Virus e Antigen, HBVeAg
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 43.7 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid, supplied in 50 mM Tris-HCl pH 8.0, 60 mM NaCl, 10 mM Glutathione, 0.25% Sarkosyl and 50% glycerol
Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
The protein contains the the HBV HBe adw immunodominant region and is fused to a GST-tag.
Background: Hepatitis B virus (HBV) is a small enveloped virus that belongs to the hepadnavirus family. The genome of the hepatitis B virus (HBV), a partially doublestranded circular DNA, has four known genes encoding the viral surface proteins (pre-S 1, pre-S2 and HBsAg), the precore (pre-C) and core (C) proteins (HBeAg and HBcAg), the DNA polymerase, the X protein. The synthesis and secretion of HbeAg are linked to the expression of the pre-C region, a small open reading frame (ORF) preceding the C region initiation codon in all HBV genomes so far sequenced. In the course of HBV infection, detection of HBeAg generally correlates with active viral replication and liver disease. The presence of hepatitis B e antigen (HBeAg) in serum indicates active viral replication in hepatocytes. HBeAg is thus a surrogate marker for the presence of hepatitis B virus DNA.
Specificity: Immunoreactive with sera of HBV-infected individuals.
Aras et al. (2005) Failure to detect hepatitis B virus in vitreous by polymerase chain reaction. Ophthalmologica. 219:93.
Mason et al. (2005) Hepatitis B virus replication in damaged endothelial tissues of patients with extrahepatic disease. Am. J.Gastroenterol. 100:972.
Leupin et al. (2005) Hepatitis B virus X protein stimulates viral genome replication via a DDB1-dependent pathway distinct from that leading to cell death. J. Virol. 79:4238.
Wu et al. (2005) RNA interference-mediated control of hepatitis B virus and emergence of resistant mutant. Gastroenterology. 128:708.
Qian et al. (2005) Cost-effective method of siRNA preparation and its application to inhibit hepatitis B virus replication in HepG2 cells. World J. Gastroenterol. 11:1297.
Zhang et al. (2005) Effects of hepatitis B virus X protein on human telomerase reverse transcriptase expression and activity in hepatoma cells. J. Lab. Clin. Med. 145:98.
Yang et al. (2002) Hepatitis B e Antigen and the Risk of Hepatocellular Carcinoma. New Engl. J. Med. 347:168.