Hepatitis B Virus Core Protein
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at 4 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 90 % (SDS-PAGE)
Form: liquid, supplied in 50 mM Tris-HCl pH 7.8, 50 mM NaCl, 0.5 mM EDTA and 50% glycerol
Immunochromatography (capture and conjugate)
Immunogen for monoclonal antibody production
Hepatitis B Virus Core Antigen contains the fragment of core protein immunodominant regions, amino acids: 1-186 fused to a His-tag. HBV core proteins are purified by proprietary chromatographic techniques.
Background: Hepatitis B Virus (HBV) is a small enveloped virus that belongs to the hepadnavirus family. The human Hepatitis B Virus (HBV) has a compact genome encoding four major overlapping coding regions: the core, polymerase, surface and X. The core protein is a structural protein. It packages its own mRNA, which is also known as the pregenomic RNA, to form the core particle. The increase of the core RNA expression has been shown to increase the viral replication rate.
Specificity: Immunoreactive with sera of HBV-infected individuals.
Le Pogam et al. (2005) Exposure of RNA templates and encapsidation of spliced viral RNA are influenced by the argininerich domain of human hepatitis B virus core antigen (HBcAg 165- 173). J. Virol. 79:1871.
Shi et al. (2004) Therapeutic polypeptides based on HBcAg (18-27) CTL epitope can induce antigen-specific CD (8) (+) CTL-mediated cytotoxicity in HLA-A2 transgenic mice. World J. Gastroenterol. 10:1222.
Szkaradkiewicz et al. (2003) HBcAg-specific cytokine production by CD4 T lymphocytes of children with acute and chronic hepatitis B. Virus Res. 97:127.