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HAV-VP1 (residues 502-605)

Hepatitis A Virus Coat Protein VP1

recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-1112 100 μg 207,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 48 kDa

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 10 mM CBB pH 9.6, 0.1% SDS and 50% glycerol)

Applications:
Recombinant HAV-VP1 Antigen may be used in ELISA and Western blots, excellent for detection of HAV with minimal specificity problems.

Description:
The E.coli derived 48 kDa recombinant protein contains the VP1 immunodominant regions, amino acids 502-605. Hepatitis A Virus VP1 protein is purified by proprietary chromatographic techniques.

Background: HAV, the prototype of the genus Hepatovirus, belongs to the family Picornaviridae. Its 7.5-kb single-stranded RNA genome bears different distinct regions: the 5' and 3' noncoding regions (NCR), the P1 region, which encodes the structural proteins VP1, VP2, VP3, and a putative VP4, and the P2 and P3 regions encoding nonstructural proteins associated with replication. Hepatitis A virus (HAV) encodes a single polyprotein which is posttranslationally processed into the functional structural and nonstructural proteins. Only one protease, viral protease 3C, has been implicated in the nine protein scissions.

Specificity: Immunoreactive with sera of HAV-infected individuals.

Selected References:
Haro et al. (2003) Liposome entrapment and immunogenic studies of a synthetic lipophilic multiple antigenic peptide bearing VP1 and VP3 domains of the hepatitis A virus: a robust method for vaccine design. FEBS Lett. 540:133.
Costa-Mattioli et al. (2002) Molecular evolution of hepatitis A virus: a new classification based on the complete VP1 protein. J. Virol. 76:9516.
Emerson et al. (2002) Identification of VP1/2A and 2C as virulence genes of hepatitis A virus and demonstration of genetic instability of 2C. J. Virol. 76:8551.
Kang et al. (2002) A proposed vestigial translation initiation motif in VP1 of hepatitis A virus. Virus Res. 87:11.
Martin et al. (1999) Maturation of the hepatitis A virus capsid protein VP1 is not dependent on processing by the 3Cpro proteinase. J. Virol. 73:6220.
Graff et al. (1999) Hepatitis A virus capsid protein VP1 has a heterogeneous C terminus. J. Virol. 73:6015.