Cytomegalo Virus Phosphoprotein 65
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 52.2 kDa +26 kDa GST
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1 mM EDTA and 50% glycerol)
The protein contains the CMV pp65 immunodominant regions, amino acids 297-510. It is fused to a 26 kDa GST-tag. The protein is purified by proprietary chromatographic technique.
Background: The E.coli derived protein contains the CMV Pp65 immunodominant regions, 297-510 aa. It is fused to a GST tag. Human cytomegalovirus (HCMV), a member of the herpesvirus family, demonstrates cell specificity for virus assembly and release. CMVpp65 (UL83), in addition to being a major constituent of dense bodies, plays a critical role in cell-mediated immunity to HCMV infection. The majority of HCMV-seropositive individuals possess CD8+ cytotoxic T-lymphocyte responses directed against pp65, and the adoptive transfer of pp65-specific cytotoxic T lymphocytes isolated from HCMV-seropositive bone marrow donors to transplant recipients has been shown to confer protection against HCMV viremia and pneumonitis. These observations have provided support for the concept of development of pp65 vaccines for prevention of HCMV infection.
Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Specificity: Immunoreactive with sera of CMV-infected individuals.
McGregor et al. (2004) Molecular, biological, and in vivo characterization of the guinea pig cytomegalovirus (CMV) homologs of the human CMV matrix proteins pp71 (UL82) and pp65 (UL83). J. Virol. 78:9872.
Browne et al. (2003) Human cytomegalovirus UL83-coded pp65 virion protein inhibits antiviral gene expression in infected cells. Proc. Natl. Acad. Sci. USA 100:11439.
Lenfant et al. (2003) Induction of HLA-G-restricted human cytomegalovirus pp65 (UL83)-specific cytotoxic T lymphocytes in HLA-G transgenic mice. J. Gen. Virol. 84:307.
Arrode et al. (2002) Cross-presentation of human cytomegalovirus pp65 (UL83) to CD8+ T cells is regulated by virus-induced, solublemediator- dependent maturation of dendritic cells. J. Virol. 76:142.
Arrode et al. (2000) Incoming human cytomegalovirus pp65 (UL83) contained in apoptotic infected fibroblasts is cross-presented to CD8 (+) T cells by dendritic cells. J. Virol. 74:10018.
Schleiss et al. (2000) Immunogenicity evaluation of DNA vaccines that target guinea pig cytomegalovirus proteins glycoprotein B and UL83. Viral. Immunol. 13:155.