Cytomegalo Virus Phosphoprotein 38
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 52.8kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1 mM EDTA and 50% glycerol)
The E.coli derived 52.8kDa recombinant protein contains the CMV Pp38 (UL80a) immunodominant regions, 117-373 amino acids and is fused to a GST-Tag at the C-terminus. The protein is purified by proprietary chromatographic technique.
Background: Human cytomegalovirus (HCMV), a member of the herpesvirus family, demonstrates cell specificity for virus assembly and release. In the viral particle this antigen is bound via S-S bridge to a lower Mr compound to form a final complex of 62 kDa. p38 is present both in the nucleus and in the cytoplasm of CMV-infected cells starting from 24 h postinfection and increasingly thereafter. Immunoelectron microscopy revealed that this antigen is mainly associated with the internal portion of viral capsids both in the nucleus and in the cytoplasm.
Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Specificity: Immunoreactive with sera of CMV-infected individuals.
Landinin et al. (1991) Human cytomegalovirus structural components: intracellular and intraviral localization of p38. Virus. Res. 19:189.