Cytomegalo Virus Phosphoprotein 38
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 52.8kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1 mM EDTA and 50% glycerol)
Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
The E.coli derived 52.8kDa recombinant protein contains the CMV Pp38 (UL80a) immunodominant regions, 117-373 amino acids and is fused to a GST-Tag at the C-terminus. The protein is purified by proprietary chromatographic technique.
Background: Human cytomegalovirus (HCMV), a member of the herpesvirus family, demonstrates cell specificity for virus assembly and release. In the viral particle this antigen is bound via S-S bridge to a lower Mr compound to form a final complex of 62 kDa. p38 is present both in the nucleus and in the cytoplasm of CMV-infected cells starting from 24 h postinfection and increasingly thereafter. Immunoelectron microscopy revealed that this antigen is mainly associated with the internal portion of viral capsids both in the nucleus and in the cytoplasm.
Specificity: Immunoreactive with sera of CMV-infected individuals.
Landinin et al. (1991) Human cytomegalovirus structural components: intracellular and intraviral localization of p38. Virus. Res. 19:189.