Epstein-Barr Virus Early Antigen
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 35.5 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 10 mM glutathione, 60 mM NaCl and 0.5% sarcosyl)
Antigen in ELISA and Western blots, excellent antigen for detection of HHV-4 (EBV) with minimal specificity problems.
The protein contains the EBV early antigen protein fragment, amino acids: 306-390. The protein is purified by proprietary chromatographic technique.
Background: Epstein-Barr virus, frequently referred to as EBV, is a member of the gamma herpesvirus family and one of the most common human viruses. The virus occurs worldwide, and most people become infected with EBV sometime during their lives. It persists in B lymphocytes for the life of the host. IgG to the early antigen appears in the acute phase and generally falls to undetectable levels after 3 to 6 months. In many people, detection of antibody to the early antigen is a sign of active infection, but 20% of healthy people may have this antibody for years.
Specificity: Immunoreactive with all sera of EBV infected individuals.
Avolio-Hunter et al. (2003) EBNA1 efficiently assembles on chromatin containing the Epstein-Barr virus latent origin of replication. Virology. 315:398.
Nikiforow et al. (2003) Cytolytic CD4 (+)-T-cell clones reactive to EBNA1 inhibit Epstein-Barr virus-induced B-cell proliferation. J. Virol. 77:12088.
Yin et al. (2003) Self-inhibition of synthesis and antigen presentation by Epstein-Barr virus-encoded EBNA1. Science 301:1371.
Jones et al. (2003) Epstein-Barr virus nuclear antigen 1 (EBNA1) induced cytotoxicity in epithelial cells is associated with EBNA1 degradation and processing. Virology. 313:663.
Kapoor et al. (2003) EBNA1 partitions Epstein-Barr virus plasmids in yeast cells by attaching to human EBNA1-binding protein 2 on mitotic chromosomes. J. Virol. 77:6946.
Deshpande et al. (2002) Lack of expression of the Epstein-Barr Virus (EBV) gene products, EBERs, EBNA1, LMP1, and LMP2A, in breast cancer cells. Lab. Invest. 82:1193.