Epstein-Barr Virus Nuclear Protein 1
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 46 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 10 mM glutathione, 60 mM NaCl and 0.5% sarcosyl)
Antigen in ELISA and Western blots, excellent antigen for detection of HHV-4 (EBV) with minimal specificity problems.
This protein does contain a GST-tag. The mosaic protein contains fragments of HHV-4 EBNA1 nuclear protein, amino acids: 1-90 and 408-498. The protein is purified by proprietary chromatographic technique.
Background: The Epstein-Barr virus (EBV), a gamma herpesvirus, persists in B lymphocytes for the life of the host. EBNA1 (Epstein-Barr virus nuclear antigen 1) is expressed in every form of EBV-related malignancy, including posttransplant lymphomas. Tumors such as nasopharyngeal cell carcinoma, Hodgkin's lymphoma, and Burkitt's lymphoma (BL) that fail to express some or all of the dominant CD8+ T-cell latent antigens still express EBNA1. A significant proportion of memory CD4+ T-cells that recognize lymphoblastoid cell lines (LCLs) are directed against the EBNA1 protein. The EBNA1 protein contains a glycine-alanine repeat that prevents proper processing and presentation through the major histocompatibility complex class I (MHC I) pathway.
Specificity: Immunoreactive with all sera of EBV infected individuals.
Avolio-Hunter et al. (2003) EBNA1 efficiently assembles on chromatin containing the Epstein-Barr virus latent origin of replication. Virology. 315:398.
Nikiforow et al. (2003) Cytolytic CD4 (+)-T-cell clones reactive to EBNA1 inhibit Epstein-Barr virus-induced B-cell proliferation. J. Virol. 77:12088.
Yin et al. (2003) Self-inhibition of synthesis and antigen presentation by Epstein-Barr virus-encoded EBNA1. Science 301:1371.
Jones et al. (2003) Epstein-Barr virus nuclear antigen 1 (EBNA1) induced cytotoxicity in epithelial cells is associated with EBNA1 degradation and processing. Virology. 313:663.
Kapoor et al. (2003) EBNA1 partitions Epstein-Barr virus plasmids in yeast cells by attaching to human EBNA1-binding protein 2 on mitotic chromosomes. J. Virol. 77:6946.
Deshpande et al. (2002) Lack of expression of the Epstein-Barr Virus (EBV) gene products, EBERs, EBNA1, LMP1, and LMP2A, in breast cancer cells. Lab. Invest. 82:1193.