p38α/ SAPK2aHis - active

Stress-activated protein kinase

human, recombinant, E. coli

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p38α/ SAPK2aHis - active PR-327 20 μg 491,05 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 42.7 kDa

Accession number: L35253

Accession number: L35253

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM Tris-HCl pH 8.5, 150 mM NaCl, 1 mM DTT and 50% glycerol)

pH: 8.5

Activity: > 200,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to myelin basic protein per min at 30 °C) N-terminal His-tagged full-length protein.

Description:
p38α/SAPK2a belongs to the family of mitogen-activated protein kinases (MAPK). Mitogen-activated protein (MAP) kinases comprise a family of ubiquitous proline-directed, proteinserine/ threonine kinases, which participate in signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. p38α/SAPK2a was discovered independently in three contexts. It was found as a tyrosine phosphoprotein present in extracts of cells treated with inflammatory cytokines; as the target of a pyridinyl imidazole drug that blocked production of tumor necrosis factor-α (TNFα) and as such was called cytokine-suppressive antiinflammatory drug-binding protein or CSBP; and as a reactivating kinase for MAP kinase-activated protein (MAPKAP) kinase-2. A variety of agents including cytokines, hormones, GPCRs, osmotic and heat shock, and other stresses activate p38 family members. The recombinant human p38α kinase (SAPK2a) was expressed in E. coli and purified by Ni-agarose chromatography. The active form was produced by phosphorylation of the purified p38a with MKK6. It is suitable for labeling p38a kinase substrates.

Selected References:
Pearson et al. (2001) Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr. Rev. 22:153.
Eckert et al. (2003) p38 Mitogen-activated protein kinases on the body surface--a function for p38 delta. J. Invest. Dermatol. 120:823.