» Sign in / Register

JNK2α2/ SAPK1aHis - active

c-jun kinase/ stress-activated protein kinase

human, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
JNK2α2/ SAPK1aHis - active PR-326 100 μg 491,05 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 49.6 kDa

Accession number: U09757

Accession number: U09757

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 8.5, 100 mM NaCl, 1 mM DTT and 50% glycerol)

pH: 8.5

Activity: > 300 U/mg (1 Unit is defined as 1 picomole phosphate transferred to c-jun 1-169-N-His- Tag per min at 30 °C) N-terminal His-tagged full-length protein.

JNK2α2/SAPK1a belongs to the family of mitogen-activated protein kinases (MAPK). Mitogen-activated protein (MAP) kinases comprise a family of ubiquitous proline-directed, proteinserine/threonine kinases, which participate in signal transduction pathways that control intracellular events including acute responses to hormones and major developmental changes in organisms. Ten separate JNK isoforms have been identified as the products of three genes with two alternative splice sites in jnk1 and jnk2 and one splice site in jnk3. The first splice site, which is present in jnk1 and jnk2, determines whether the resulting protein will be the α or the β isoform. The second splice site, which is present in all three jnk genes, dictates whether the p46 or p54 isoforms are encoded. The smaller p46 JNKs are referred to as JNK1α1, JNK1β1, JNK2α1, JNK2β1, and JNK3α1. The p54 JNK proteins are referred to as JNK1α2, JNK1β2, JNK2α2, JNK2β2, and JNK3α2. The recombinant human JNK2α2 was expressed in E. coli and purified by Ni-agarose chromatography. It is suitable for labeling JNK2α2 kinase substrates.

Selected References:
Kallunki et al. (1994) JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation. Genes Dev. 15:2996.
Su et al. (1994) JNK is involved in signal integration during costimulation of T lymphocytes. Cell. 77:727.
Dreskin et al. (2001) Isoforms of Jun kinase are differentially expressed and activated in human monocyte/macrophage (THP-1) cells. J. Immunol. 166:5646.