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p44/ERK1His - active

Extracellular signal-regulated kinase/ mitogen-activated protein kinase

human, recombinant, E. coli

Product Cat. No. Amount Price (EUR) Buy / Note
p44/ERK1His - active PR-322 10 μg 491,05 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 44.5 kDa

Accession number: NM_002746

Accession number: NM_002746

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 8.5, 150 mM NaCl, 1 mM DTT and 50% glycerol)

pH: 8.5

Activity: > 200,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to myelin basic protein per min at 30°C).

Description:
N-terminal His-tagged full-length protein. Members of the MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPK families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. MAPK pathways relay, amplify and integrate signals from a diverse range of stimuli and elicit an appropriate physiological response including cellular proliferation, differentiation, development, inflammatory responses and apoptosis in mammalian cells. Activated ERK can enter the nucleus and phosphorylate transcription factors providing the link between cell surface receptor-mediated events and nuclear induction of gene expression. In the nucleus activated ERK promotes the transcription and the activity of transcription factors c-fos, c-myc, c-jun and p21. The recombinant human ERK1 was expressed in E. coli and purified by Ni-agarose chromatography. It is suitable for labeling ERK1 substrates. The highly active form is produced by phosphorylation of the protein in vitro with MEK1.

Selected References:
Zhang et al. (2002) MAPK signal pathways in the regulation of cell proliferation in mammalian cells. Cell Res. 12:9.
Ajenjo et al. (2004) Subcellular localization determines the protective effects of activated ERK2 against distinct apoptogenic stimuli in myeloid leukemic cells. J. Biol. Chem. 279:32813.