Casein Kinase 2, α-subunit
Zea mays, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 39.2 kDa
Accession number: X61387
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.5, 500 mM NaCl, 1 mM DTT, 500 μM PMSF and 50% glycerol)
Activity: > 1 U/mg (1 Unit is defined as 1 micromole phosphate transferred to synthetic peptide RRRDDDSDDD (cat.# PE-207) per min at 30 °C).
Casein kinase 2 (CK2) is a ubiquitous pleiotropic proliferation-associated serine/threonine protein kinase. Similar to protein kinase A, CK2 is a tetramer made up from two different subunits α2β2. The catalytic α-subunit corresponds to the C-subunit of PKA, the non-catalytic β-subunit is unique and differs from the R-subunit of PKA in all known features. The recombinant protein kinase CK2α from Zea mays was expressed in E. coli as a non fusion protein and purified by several chromatography steps (phosphocellulose, Superose 6, Resource Q). It is less prone to degradation than the human counterpart. The α-subunit is constitutively active and suitable for labeling casein kinase 2 α substrates. It is shown to be extremely salt sensitive, the highest activity is shown without salt.
Boldyreff et al. (1993) Expression and characterization of a recombinant maize CK-2 alpha subunit. Biochim. Biophys. Acta 1173:32.
Dobrowolska et al. (1991) Cloning and sequencing of the casein kinase 2 alpha subunit from Zea mays. Biochim. Biophys. Acta. 1129:139.