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CHK2His - active

Checkpoint Kinase 2

human, recombinant, Sf9 insect cells

Product Cat. No. Amount Price (EUR) Buy / Note
CHK2His - active PR-313 20 μg 491,05 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 65.4 kDa

Accession number: BC0004207

Accession number: BC0004207

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 10 mM DTT, 0.1 mM EGTA, 270 mM sucrose and protease inhibitor cocktail (Roche))

pH: 7.5

Activity: > 100,000 U/mg (1 Unit is defined as 1 picomole phosphate transferred to synthetic peptide KKKVSRSGLYRSPSMPENLNRPR (cat.# PE-205) per min at 30 °C).

C-terminal His-tagged full-length protein. Check- point kinase 2 (CHK2) is emerging as a key mediator of diverse cellular responses to genotoxic stress, guarding the integrity of the genome throughout eukaryotic evolution. Recent studies show the fundamental role of CHK2 in the network of genomesurveillance pathways that coordinate cell-cycle progression with DNA repair and cell survival or death. Defects in CHK2 contribute to the development of both hereditary and sporadic human cancers, and earmark this kinase as a candidate tumour suppressor and an attractive target for drug discovery. The recombinant human CHK2 was expressed in Sf9 insect cells and purified by Ni-NTA agarose chromatography. It is suitable for labeling CHK substrates.

Selected References:
Bartek et al. (2001) CHK2 kinase--a busy messenger. Nat. Rev. Mol. Cell Biol. 12:877.
Gire et al. (2004) DNA damage checkpoint kinase Chk2 triggers replicative senescence. EMBO J. 23:2554.