muscle and microspikes Ras, related ras viral oncogene homolog 3
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Accession number: NM_012219
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 25 mM HEPES pH 7.5, 50 mM NaCl, 5 mM MgCl2, 1 μM GDP and 50% glycerol)
Activity: GTPγS binding: >750 mmol/mol.
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. The Ras family of GTP-binding proteins consists of the classical Ras (Ha-, K-, and N-), R-Ras, Rap1, Rap2, Ral, Rheb, Rin, Rit, TC21/R-Ras2, and M-Ras/R-Ras3 (M-Ras). Stimulation of Ba/F3-Fms cells with either IL-3 or CSF-1 resulted in efficient activation of both K-Ras 4B and M-Ras. Stimulation of fibroblasts with EGF also led to more efficient activation of K-Ras 4B than of H-Ras or N-Ras, and the activation of M-Ras was very strong. An S27N mutant of M-Ras, like the analogous H-Ras S17N mutant, was a dominant inhibitor of activation of the c-fos promoter by constitutively active Src Y527F, suggesting that M-Ras and p21 Ras shared guanine nucleotide exchange factors and are likely to be activated in parallel. M-Ras (Q71L) is a constitutive active mutant of M-Ras.
Ohba et al. (2000) Regulatory proteins of R-Ras, TC21/R-Ras2, and M-Ras/R-Ras3. J. Biol. Chem. 27:20020.
Ehrhardt et al. (2004) Distinct Mechanisms Determine the Patterns of Differential Activation of H-Ras, N-Ras, K-Ras 4B, and M-Ras by Receptors for Growth Factors or Antigen. Mol. Cell. Biol. 24:6311.
Ehrhardt et al. (1999) M-Ras, a widely expressed 29-kD homologue of p21 Ras: expression of a constitutively active mutant results in factorindependent growth of an interleukin-3-dependent cell line. Blood. 94:2433.