H-Ras (G12V)

For general laboratory use.

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 20.97 kDa (189 amino acids)

Accession number: NP_005334

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 64 mM Tris-HCl pH 7.2, 10 mM MgCl₂ and 5 mM DTE)

Description:
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). The mutation G12V leads to elimination of the intrinsic GTPase activity. H-Ras (G12V) is effective in activation of PI3K and PKB, whereas N-Ras and K-Ras are more potent towards MAP kinase.

BIOZ Product Citations:

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Selected References:
Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J Biol. Chem. 280:22856.
Wittinghofer et al. (2000) Ras - a molecular switch involved in tumor formation. Angew. Chem. Int. Ed. 39:4192.
Li et al. (1997) Uncoupling of membrane ruffling and pinocytosis during Ras signal transduction. J. Biol. Chem. 272:10337.
Pacold et al. (2000) Crystal structure and functional analysis of Ras binding to its effector Phosphoinositide 3-kinase γ. Cell 103:931.
Li et al. (2004) Transformation Potential of Ras Isoforms Correlates with Activation of Phosphatidylinositol 3-Kinase but Not ERK. J. Biol. Chem. 279:37398.