Harvey rat sarcoma viral oncogene homolog
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 21.3 kDa (189 amino acids)
Accession number: NP_005334
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 64 mM Tris-HCl pH 7.2, 10 mM MgCl2 and 5 mM DTE)
Ras proteins are members of the superfamily of small GTP-binding proteins that function as molecular switches controlling a variety of signaling and transport pathways. H-Ras is one of the classic human Ras proteins (H-, N-, K-Ras4A, and K-Ras4B). The mutation Q61L results in a decreased GTPase activity as well as increased GDP/GTP exchange. This mutant constitutively activates the Ras-signaling pathway. Protein preparation is 77% GDP- and 23% GTP-loaded, measured by HPLC.
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
Sasazuki et al. (2005) Transformation by Oncogenic RAS Sensitizes Human Colon Cells to TRAIL-induced Apoptosis by Up-regulating Death Receptor 4 and Death Receptor 5 through a MEK-dependent Pathway. J Biol. Chem. 280:22856.
Wittinghofer et al. (2000) Ras - a molecular switch involved in tumor formation. Angew. Chem. Int. Ed. 39:4192.
Frech et al. (1994) Role of glutamine-61 in the hydrolysis of GTP by p21H-ras: an experimental and theoretical study. Biochemistry 33:3237.