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Ran - G19V/Q69L

Ras-related nuclear protein, TC4

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-214 50 μg 212,18 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 24.0 kDa (216 amino acids)

Accession number: NM_006325

Accession number: NM_006325

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM KH2PO4/K2HPO4 pH 7.0, 6.5 mM DTE, 150 mM KCl and 5 mM MgCl2)

Ran belongs to the Ras superfamily of GTP binding proteins. It acts in cellular processes such as nuclear transport, mitotic spindle assembly, and post-mitotic nuclear assembly. The mutation Q69L abolishes GTP hydrolysis. The mutation G19V results in a gain-of-function mutant that is not, however, sensitive to the exchange factor RCC1. Protein preparation is 19% GDP-loaded, measured by HPLC.

Selected References:
Siebrasse et al. (2002) Reconstitution of nuclear protein export in isolated nuclear envelopes. J. Cell Biol. 158:849.
Lounsbury KM (1996) Mutations within Ran/TC4 GTPase. Effects on regulatory factor interactions and subcellular localization. J. Biol. Chem. 271:32834.
Palacios et al. (1996) Ran/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus. J. Cell Biol. 133:485.
Clarke et al. (1995) Regulation of Cdc2/cyclin B activation by Ran, a Ras-related GTPase. J. Cell Sci. 108:1217.