P21 activated kinase 1, Cdc42/Rac interactive binding region (AA 56-141), GST-tagged
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles, aliquot in convenient format upon first thawing
Shelf Life: 12 months
Molecular Weight: 35 kDa (inclusive GST-tag)
Purity: > 90 % (SDS PAGE), 30-50 % degradation (Please note: effective concentration of full-length polypeptide takes into account protein degradation that commonly accounts for 30-50 % of total protein)
Form: liquid (Supplied in PBS)
Members of the Rac/Rho subfamily of small GTPases or G-proteins are key regulators of numerous processes related to cytoskeletal dynamics. For example, Rac1/2 and cdc42 (and other closely related members) regulate cell migration, contraction and cell volume, among other features, through the control of the cortical actin cytoskeleton (1,2). Cdc42 is furthermore a major player in the establishment of a polarized phenotype in numerous specialized cell types (3) whereas Rac is a critical regulator of NADPH oxidases and concomitant ROS production (4). In common with most other small G-proteins, cellular Rac and Cdc42 cycle between an inactive GDP-bound and an active GTP bound state. The CRIB domain of the Rac-effector PAK1 discriminates between these two conformations and can be used to isolate active Rac/Cdc42GTP complexes out of cell extracts (5).
Heasman et al. (2008) Nat Rev Mol Cell Biol. 9:690.
Ladwein et al. (2006) FEBS Lett. 582:2066.
Sinha et al. (2008) Cell Signal. 20 381:1927.
Bokoch et al. (2006) Antioxid Redox Signal. 8:1533.
Ogita et al. (2006) Meth Enzymol. 406:415.
Bondeva et al. (2011) Advanced glycation end products inhibit adhesion ability of differentiated podocytes in a neuropilin 1-dependent manner. American Journal of Physiology - Renal Physiology 301:852.