Rhotekin Ras Binding Domain, from the Japanese
human, recombinant, E. coli
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PR-367 | 50 μg | 271,40 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Accession number: NM_033046
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 7.5, 100 mM NaCl and 2 mM DTE)
Description:
Rhotekin (from the Japanese teki, meaning target) is an effector of the Rho-GTPases that was previously identified in mice. GST-RhotekinRBD is a glutathione S-transferase (GST)-fusion protein of the Rho-binding domain (RBD) of Rhotekin. The N-terminal peptide of Rhotekin contains the amino acids 1-89 comprising the Rho-binding domain between amino acids 7 and 89. The GST-Tag facilitates the protein`s application in typical GST pull down assays.
Selected References:
Reynaud et al. (2000) The PDZ protein TIP-1 interacts with the Rho effector Rhotekin and is involved in Rho signaling to the serum response element. J. Biol. Chem. 275:33962.
Reid et al. (1996) Rhotekin, a new putative target for Rho bearing homology to the serine/threonine kinase, PKN, and rhodophilin in the Rho-binding domain. J. Biol. Chem. 271:13556.
