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RhotekinRBDGST

Rhotekin Ras Binding Domain, from the Japanese

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-367 50 μg 212,18 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Accession number: NM_033046

Accession number: NM_033046

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM Tris-HCl pH 7.5, 100 mM NaCl and 2 mM DTE)

Description:
Rhotekin (from the Japanese teki, meaning target) is an effector of the Rho-GTPases that was previously identified in mice. GST-RhotekinRBD is a glutathione S-transferase (GST)-fusion protein of the Rho-binding domain (RBD) of Rhotekin. The N-terminal peptide of Rhotekin contains the amino acids 1-89 comprising the Rho-binding domain between amino acids 7 and 89. The GST-Tag facilitates the protein`s application in typical GST pull down assays.

Selected References:
Reynaud et al. (2000) The PDZ protein TIP-1 interacts with the Rho effector Rhotekin and is involved in Rho signaling to the serum response element. J. Biol. Chem. 275:33962.
Reid et al. (1996) Rhotekin, a new putative target for Rho bearing homology to the serine/threonine kinase, PKN, and rhodophilin in the Rho-binding domain. J. Biol. Chem. 271:13556.