Wiskott-Aldrich Syndrome Protein
human, recombinant, E. coli,
Recognized GTPases: Cdc42, TC10,TCL
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Accession number: U12707
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 7.5, 100 mM NaCl and 2 mM DTE)
The Wiskott-Aldrich syndrome protein (WASP) is the founding member of a family of proteins that have emerged as crucial effectors of Rho GTPases and activators of the cytosceletalorganizing complex Arp2/3. WASP has been shown to be intimately involved in many pathways that influence the function of the immune system. WASP-121 comprises the amino acids 201-321 of the native WASP sequence. This domain is essential for the binding of Cdc42 to WASP. The GST-Tag facilitates the protein`s application in typical GST pull down assays.
Thrasher (2002) WASp in immun-system organization and function. Nature Reviews Immunology 2:635.
Rudolph et al. (1998) The Cdc42/Rac interactive binding region of the Wiskott-Aldrich-Syndrome Protein (WASP) is necessary but not sufficient for tight binding to Cdc42 and structure formation. J. Biol. Chem. 273:18067.