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RafRBDGST (c-Raf)

Ras Binding Domain of Raf, c-Raf 1 Kinase

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-366 300 μg 224,39 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 42 kDa

Accession number: X03484 / P04049

Accession number: X03484 / P04049

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 32 mM Tris-HCl pH 7.6, 200 mM NaCl and 5 mM MgCl2)

Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phosphorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.

Selected References:
Eing et al. (2002) Quantification of the Raf-C1 interaction with solidsupported bilayers. ChemBioChem 3:190.
Emerson et al. (1996) Structure of Ras-binding domain of c-Raf-1 as determined by NMR spectroscopy and identification of the region that interacts with Ras. Drug Des. Discov. 13:83.
Nasser et al. (1995) The 2.2 crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue. Nature 375:554.