Ras Binding Domain of Raf, c-Raf 1 Kinase
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 9.4 kDa (50 - 132 amino acids)
Accession number: X03484 / P04049
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 32 mM Tris-HCl pH 7.6, 200 mM NaCl, 5 mM MgCl2 and 5 mM DTT)
Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phosphorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf.
Eing et al. (2002) Quantification of the Raf-C1 interaction with solidsupported bilayers. ChemBioChem 3:190.
Emerson et al. (1996) Structure of Ras-binding domain of c-Raf-1 as determined by NMR spectroscopy and identification of the region that interacts with Ras. Drug Des. Discov. 13:83.
Nasser et al. (1995) The 2.2 crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue. Nature 375:554.
Bahk et al. (2008) A Cross-talk between oncogenic Ras and tumor suppressor PTEN through FAK Tyr861 phosphorylation in NIH/3T3 mouse embryonic fibroblasts. Biochemical and Biophysical Research Communications 377 (4):1199-1204.