Interleukin 9, T-cell growth factor P40
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 14 kDa
Accession number: P15248
Accession number: P15248
Purity: > 98.5 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilised IL-9 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.2 ng/ml, corresponding to a specific activity 5 x 106 IU/mg, determined by the dosedependent stimulation of human MO7e cells.
Interleukin-9 (IL-9) has been strongly implicated in the pathogenesis of asthma, including the overproduction of mucus, in humans and in animal models. IL-9 is known to regulate many cell types involved in T-helper type 2 responses classically associated with asthma, including B- and T-lymphocytes, mast cells, eosinophils and epithelial cells. Recombinant human IL-9 produced in E. coli is a single, non-glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of 14 kDa. IL-9 is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IL-9.
Pilette et al. (2002) Oxidative burst in lipopolysaccharide-activated human alveolar macrophages is inhibited by interleukin-9. Eur. Respir. J. 20:1198.
Reader et al. (2003) Interleukin-9 induces mucous cell metaplasia independent of inflammation. Am. J. Respir. Cell. Mol. Biol. 28:664.
Yang et al. (2004) Epstein-Barr virus (EBV)-encoded RNA promotes growth of EBV-infected T cells through interleukin-9 induction. Cancer Res. 64:5332.
McNamara et al. (2004) Interleukin 9 production in the lungs of infants with severe respiratory syncytial virus bronchiolitis. Lancet. 363:1031.
Hoebee et al. (2004) Influence of promoter variants of interleukin-10, interleukin-9, and tumor necrosis factor-alpha genes on respiratory syncytial virus bronchiolitis. J. Infect. Dis. 189:239.
Panzner et al. (2003) Marked up-regulation of T lymphocytes and expression of interleukin-9 in bronchial biopsies from patients With chronic bronchitis with obstruction. Chest. 124:1909.
Nishimura et al. (2004) Elevated interleukin-9 receptor expression and response to interleukins-9 and -7 in thymocytes during radiationinduced T-cell lymphomagenesis in B6C3F1 mice. J. Cell. Physiol. 198:82.
Fischer et al. (2003) Increased serum levels of interleukin-9 correlate to negative prognostic factors in Hodgkin's lymphoma. Leukemia. 17:2513.