Interleukin 4, B-cell stimulatory factor
murine, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 13.5 kDa
Accession number: P07750
Accession number: P07750
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Solubility: It is recommended to reconstitute the lyophilized IL-4 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 2 ng/ml, determined by the dosedependent proliferation of murine HT-2 cells.
The anti-inflammatory cytokine Interleukin-4 is involved in the regulation of inflammatory responses by promoting the differentiation of naïve T-cells to T helper type 2 (Th2) cells. A key anti-inflammatory action of IL-4 results from its ability to inhibit the release of pro-inflammatory cytokines by innate immune cells and to upregulate the synthesis of IL-1 receptor antagonist. IL-4 has been demonstrated to be a potent cofactor for B and T lymphocyte proliferation and differentiation. Recombinant murine IL-4 produced in E. coli is a single, non-glycosylated form of murine IL-4 polypeptide chain containing 120 amino acids and having a molecular mass of 13.5 kDa. IL-4 is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of IL-4.
Wurster et al. (2002) Interleukin-4-mediated protection of primary B cells from apoptosis through Stat6-dependent up-regulation of BclxL. J. Biol. Chem. 277:27169.
Nolan et al. (2004) Role of interleukin-4 in regulation of age-related inflammatory changes in the hippocampus. J. Biol. Chem. 280:9354.
McCurdy et al. (2004) Modified vaccinia virus Ankara immunization protects against lethal challenge with recombinant vaccinia virus expressing murine interleukin-4. J. Virol. 78:12471.
Yatsenko et al. (2004) Alternative splicing of murine interleukin-4 mRNA. Bull. Exp. Biol. Med. 137:179.
Walz et al. (2002) A murine interleukin-4-Ig fusion protein regulates the expression of Th1- and Th2-specific cytokines in the pancreas of NOD mice. Horm. Metab. Res. 34:561.
Ghiasi et al. (2001) Recombinant herpes simplex virus type 1 expressing murine interleukin-4 is less virulent than wild-type virus in mice. J. Virol. 75:9029.
Yoshikawa et al. (2000) Immune gene therapy of experimental mouse brain tumor with adenovirus-mediated gene transfer of murine interleukin-4. Cancer Immunol. Immunother. 49:23.
Missol-Kolka et al. (1998) Combined therapy of B16 (F10) murine melanoma using E. coli cytosine deaminase gene and murine interleukin-4 gene. Neoplasma. 45:305.