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Leptin - triple mutant (L39A/D40A/F41A)

Obesity Factor

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-484 100 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 16 kDa

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilised (Leptin is lyophilised from a 1 mg/ml solution containing 0.0045 mM NaHCO3)

Solubility: It is recommended to reconstitute the lyophilised Leptin mutant in sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. At low concentrations addition of a carrier protein (0.1% HSA or BSA) is suggested.

Activity: This Leptin triple mutant is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Human Leptin, one polypeptide chain containing 146 amino and additional Ala at the N-terminus and having a molecular mass of {tab??}16 kDa, was mutated, resulting in a L39A/D40A/F41A mutant. Leptin mutant was purified by proprietary chromatographic techniques.

Endotoxin: Less than 0.1 ng/μg (IEU/μg) of Leptin.

Selected References:
LaMarca et al. (2004) Recombinant human leptin in women with hypothalamic amenorrhea. N. Engl. J. Med. 351:2343.
Welt et al. (2004) Recombinant human leptin in women with hypothalamic amenorrhea. N. Engl. J. Med. 351:987.
Cochran et al. (2004) Efficacy of recombinant methionyl human leptin therapy for the extreme insulin resistance of the Rabson- Mendenhall syndrome. J. Clin. Endocrinol. Metab. 89:1548.
Zeyl et al. (2004) Interactions between temperature and human leptin physiology in vivo and in vitro. Eur. J. Appl. Physiol. 92:571.
Lagarde et al. (2003) Development of radioimmunoassay for the measurement of human leptin in serum. Nucl. Med. Rev. Cent. East. Eur. 6:105.
Wang et al. (2003) Mice body weight gain is prevented after naked human leptin cDNA transfer into skeletal muscle by electroporation. J. Gene Med. 5:966.