ovine (sheep), recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 16 kDa
Accession number: Q28603
Accession number: Q28603
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilised (Leptin is lyophilised from a 1 mg/ml solution containing 0.0045 mM NaHCO3)
Solubility: It is recommended to reconstitute the lyophilised Leptin in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.33-0.05 ng/ml, calculated by the leptin-dependent stimulation of human OB-R transfected murine BaF3 indicator cells.
Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Ovine Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.1 kDa. Recombinant Leptin is purified by proprietary chromatographic techniques.
Endotoxin: Less than 0.1 ng/μg (IEU/μg) of Leptin.
Laborde et al. (2004) Overexpression of ovine leptin in Pichia pastoris: physiological yeast response to leptin production and characterization of the recombinant hormone. Yeast. 21:249.
Howe et al. (2002) The late gestation increase in circulating ACTH and cortisol in the fetal sheep is suppressed by intracerebroventricular infusion of recombinant ovine leptin. J. Endocrinol. 174:259.
Amstalden et al. (2002) Central infusion of recombinant ovine leptin normalizes plasma insulin and stimulates a novel hypersecretion of luteinizing hormone after short-term fasting in mature beef cows. Biol. Reprod. 66:1555.
Morrison et al. (2002) Effect of intravenous infusion of recombinant ovine leptin on feed intake and serum concentrations of GH, LH, insulin, IGF-1, cortisol, and thyroxine in growing prepubertal ewe lambs. Domest. Anim. Endocrinol. 22:103.
Newby et al. (2001) Effects of recombinant ovine leptin on in vitro lipolysis and lipogenesis in subcutaneous adipose tissue from lactating and nonlactating sheep. J. Anim. Sci. 79:445.
Kauter et al. (2000) Adrenaline, insulin and glucagon do not have acute effects on plasma leptin levels in sheep: development and characterisation of an ovine leptin ELISA. J. Endocrinol. 166:127.