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Obesity Factor

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-480 1 mg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Accession number: P41159

Accession number: P41159

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilised (Leptin is lyophilised from a 1 mg/ml solution containing 0.0045 mM NaHCO3)

Solubility: The lyophilised Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. Recombinant Human Leptin produced in E. coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa. Recombinant Leptin is purified by proprietary chromatographic techniques.

Selected References:
LaMarca et al. (2004) Recombinant human leptin in women with hypothalamic amenorrhea. N. Engl. J. Med. 351:2343.
Welt et al. (2004) Recombinant human leptin in women with hypothalamic amenorrhea. N. Engl. J. Med. 351:987.
Cochran et al. (2004) Efficacy of recombinant methionyl human leptin therapy for the extreme insulin resistance of the Rabson- Mendenhall syndrome. J. Clin. Endocrinol. Metab. 89:1548.
Zeyl et al. (2004) Interactions between temperature and human leptin physiology in vivo and in vitro. Eur. J. Appl. Physiol. 92:571.
Lagarde et al. (2003) Development of radioimmunoassay for the measurement of human leptin in serum. Nucl. Med. Rev. Cent. East. Eur. 6:105.
Wang et al. (2003) Mice body weight gain is prevented after naked human leptin cDNA transfer into skeletal muscle by electroporation. J. Gene Med. 5:966.