human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 18.6 kDa
Accession number: P22301
Accession number: P22301
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (Interleukin-10 was lyophilized from a 1 mg/ml solution in PBS, pH 7.4)
Solubility: It is recommended to reconstitute the lyophilized IL-10 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)
Activity: ED50: < 2 ng/ml, corresponding to a specific activity 1.5 x 107 IU/mg, determined by the dosedependent co-stimulation with murine IL-4 of MC-9 cells.
Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cellderived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. In humans IL-10 is produced by activated CD8+ peripheral blood T-cells, by T-helper CD4+ T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium. The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10. Recombinant human Interleukin-10 produced in E.coli is a single non-glycosylated polypeptide chains containing 161 amino acids each.
Amino Acid Sequence:
MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N.
Azar et al. (2004) Increased secretion patterns of interleukin-10 and tumor necrosis factor-alpha in cervical squamous intraepithelial lesions. Hum. Pathol. 35:1376.
Schwerbrock et al. (2004) Interleukin 10-deficient mice exhibit defective colonic Muc2 synthesis before and after induction of colitis by commensal bacteria. Inflamm. Bowel Dis. 10:811.
Luik et al. (2004) Autoregulatory role of interleukin-10 in hepatitis C patients treated with IFN-alpha. J. Interferon Cytokine Res. 24:585.
Burgess et al. (2004) Longitudinal decline in lung function: evaluation of interleukin-10 genetic polymorphisms in firefighters. J. Occup. Environ. Med. 46:1013.
Salvati et al. (2005) Recombinant human interleukin 10 suppresses gliadin dependent T cell activation in ex vivo cultured coeliac intestinal mucosa. Gut. 54:46.
Bouton et al. (2004) Costimulation with interleukin-4 and interleukin-10 induces mast cell apoptosis and cell-cycle arrest: the role of p53 and the mitochondrion. Exp. Hematol. 32:1137.