Bone Morphogenetic Protein-2
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 26 kDa
Accession number: P12643
Accession number: P12643
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (BMP-2 was lyophilized from a 1 mg/ml solution containing 20 mM sodium avetate pH 4.0)
Solubility: It is recommended to reconstitute the lyophilized BMP-2 in sterile 20mM acetc Acid not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: <50 ng/ml, determined by the cytolysis of MC3T3-E1 cells. Calcium uptake in mouse muscle tissue >1000μg/mg. In mouse muscle myoblast C2C12 cell line, activity was found to be 1x105 U/mg protein.
The Bone Morphogenetic Protein-2 belongs to the bone-growth regulatory factors that are members of the transforming growth factor-beta (TGFβ) superfamily of proteins. BMPs are widely expressed in the mammalian nervous system, where they exert trophic effects on several neuronal populations. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. Bone morphogenetic protein-2 promote the differentiation of GABAergic neurons, especially of the calbindin-positive subpopulation, the subset of projecting striatal neurons that degenerates in Huntington's disease. Recombinant Human Bone Morphogenetic protein-2 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 26 kDa. BMP-2 is purified by proprietary chromatographic techniques.
Gratacos et al. (2001) Bone morphogenetic protein-2, but not bone morphogenetic protein-7, promotes dendritic growth and calbindin phenotype in cultured rat striatal neurons. Neuroscience. 104:783.
Reppe et al. (2004) Butyrate response factor 1 is regulated by parathyroid hormone and bone morphogenetic protein-2 in osteoblastic cells. Biochem. Biophys. Res. Commun. 324:218.
Kim et al. (2004) Bone morphogenetic protein-2-induced alkaline phosphatase expression is stimulated by Dlx5 and repressed by Msx2. J. Biol. Chem. 279:50773.
Seeherman et al. (2004) Recombinant human bone morphogenetic protein-2 delivered in an injectable calcium phosphate paste accelerates osteotomy-site healing in a nonhuman primate model. J. Bone Joint Surg. Am. 86:1961.
Takagi et al. (2004) Effects of bone morphogenetic protein-2 and transforming growth factor beta1 on gene expression of transcription factors, AJ18 and Runx2 in cultured osteoblastic cells. J. Mol. Histol. 35:81.
Kaden et al. (2004) Expression of bone sialoprotein and bone morphogenetic protein-2 in calcific aortic stenosis. J. Heart Valve Dis. 13:560.
Mummaneni et al. (2004) Contribution of recombinant human bone morphogenetic protein-2 to the rapid creation of interbody fusion when used in transforaminal lumbar interbody fusion: a preliminary report. Invited submission from the Joint Section Meeting on Disorders of the Spine and Peripheral Nerves, March 2004. J. Neurosurg. Spine. 1:19.