human, recombinant, Chinese Hamster Ovary (CHO) cells
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 18.3 kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (IL-12 was lyophilized from 0.2 μm filtered 0.5mg/ml PBS pH 7.4)
Solubility: It is recommended to reconstitute the lyophilized IL-12 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA)
Activity: The ED50 as determined by the dose-dependant stimulation of the IFN-gamma by human PBMC co-stimulated with IL-12 was found to be 5ng/ml.
Human recombinant Interleukin-12 produced in CHO is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a Molecular Weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits. Interleukin-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity. The IL-12 is purified by proprietary chromatographic techniques.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and found to be Arg-Asn-Leu-Pro-Val at the p35 subunit and Ile-Trp-Glu-Leu-Lys at the p40 subunit.
Sorensen et al. (2010) IL-12 suppresses vascular endothelial growth factor receptor 3 expression on tumor vessels by two distinct IFN-gamma-dependent mechanisms. J. Immunol. 184:1858.
Feng et al. (2005) Morphine withdrawal sensitizes mice to lipopolysaccharide: elevated TNF-alpha and nitric oxide with decreased IL-12. J Neuroimmunol. 164:57.
Torre et al. (2002) Transforming growth factor-beta, interleukin (IL)-18, and IL-12: effect on the clinical course and complications of Plasmodium falciparum malaria. J Infect Dis. 187:338.
Scott (1993) IL-12: initiation cytokine for cell-mediated immunity. Science 260:496.