Keratinocyte Growth Factor 2
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Accession number: O15520
Accession number: O15520
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (KGF-2 is lyophilized from a 1 mg/ml solution containing 5 mM Sodium phosphate buffer pH 7.4 and 80 mM NaCl)
Solubility: It is recommended to reconstitute the lyophilized KGF in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.5 ng/ml, corresponding to a specific activity 2 x 106 IU/mg, stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake).
Keratinocyte growth factor-2 (KGF-2), also described as fibroblast growth factor-10 (FGF-10), is a member of the fibroblast growth factor family. KGF-2 shares 57% sequence homology to KGF-1 (FGF-7). It selectively induces epithelial cell proliferation, differentiation and migration. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF. Recombinant human KGF-2 (FGF-10) produced in E. coli is a single, non-glycosylated polypeptide chain containing 170 amino acids and having a molecular mass of 19.3 kDa. KGF-2 is highly related to KGF-1 (FGF-7), it binds to the same receptor as KGF-1 and shares 57% sequence homology. Recombinant KGF-2 is purified by proprietary chromatographic techniques.
Kovacs et al. (2005) Immunohistochemical analysis of keratinocyte growth factor and fibroblast growth factor 10 expression in psoriasis. Exp. Dermatol. 14:130.
Entesarian et al. (2005) Mutations in the gene encoding fibroblast growth factor 10 are associated with aplasia of lacrimal and salivary glands. Nat. Genet. 37:125.
Hyatt et al. (2004) FGF-10 induces SP-C and Bmp4 and regulates proximal-distal patterning in embryonic tracheal epithelium. Am. J. Physiol. Lung. Cell. Mol. Physiol. 287:L1116.
Lovinescu et al. (2003) Roles of FGF-10 on the development of diathrodial limb joints. Penn. Dent. J. (Phila.). 103:5.
Asaki et al. (2004) Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo. Mol. Cell. Endocrinol. 218:119.
Yucel et al. (2004) Anatomical studies of the fibroblast growth factor- 10 mutant, Sonic Hedge Hog mutant and androgen receptor mutant mouse genital tubercle. Adv. Exp. Med. Biol. 545:123.