Interleukin-6, B-cell stimulatory factor
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped at ambient temperature
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 21 kDa
Accession number: P05231
Accession number: P05231
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: lyophilized (IL-6 is lyophilized from a concentrated PBS pH 7.4 solution)
Solubility: It is recommended to reconstitute the lyophilized IL-6 in bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Activity: ED50: < 0.1 ng/ml, corresponding to a specific activity of 1 x 107 IU/mg, determined by the dosedependent stimulation of murine 7TD1 cells.
Recombinant human IL-6 produced in E. coli is a single, non-glycosylated polypeptide chain containing 184 amino acids. IL-6 is a variably glycosylated protein produced by many different cells, but the main sources in vivo are stimulated monocytes/macrophages, fibroblasts, and vascular endothelial cells, indicative of its role in the modulation of the immune system. Other cells known to express IL-6 include keratinocytes, osteoblasts, T-cells, B-cells, neutrophils, eosinophils, mast cells, smooth muscle cells, and skeletal muscle cells. Typical stimuli for IL-6 production are IL-1, TNF-α, and bacterial endotoxin. IL-6 is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be
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