IL-1 β

Interleukin 1 beta

murine, recombinant, E. coli

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IL-1 β PR-459 10 μg 253,00 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped at ambient temperature

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 17.5 kDa

Accession number: P10749

Accession number: P10749

Purity: > 95 % (SDS-PAGE, RP-HPLC)

Form: lyophilized

Solubility: It is recommended to reconstitute the lyophilized IL-1 β in sterile bidest H2O not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Activity: ED50: < 0.002 ng/ml, corresponding to a specific activity of 5 x 108 IU/mg, determined by the dose-dependent stimulation of murine D10S cells.

Description:
Interleukin-1 beta produced in E.coli is a non-glycosylated polypeptide chain containing 153 amino acids. Interleukin 1 β (IL-1 β) is a soluble factor produced by monocytes, macrophages, and other cells which activates T-lymphocytes and potentiates their response to mitogens or antigens. Murine macrophages display a transition from IL-1 β to IL-1 α production during maturation of monocytes into inflammatory macrophages. The synthesis of IL-1 can be induced by other cytokines including TNF-α, IFN-α , IFN-β and IFN-γ and also by bacterial endotoxins , viruses, mitogens, and antigens. In human skin fibroblasts IL-1 α and TNF-α induce the synthesis of IL-1 β. In pheochromocytoma cells, NGF induces the synthesis of IL-1. IL-1 β is purified by proprietary chromatographic techniques.

Selected References:
Godambe et al. (1995) A novel cis-acting element required for lipopolysaccharide-induced transcription of the murine interleukin-1 beta gene. Mol. Cell. Biol. 15:112.
Godambe et al. (1994) Upstream NFIL-6-like site located within a DNase I hypersensitivity region mediates LPS-induced transcription of the murine interleukin-1 beta gene. J. Immunol. 153:143.
Godambe et al. (1994) An NFIL-6 sequence near the transcriptional initiation site is necessary for the lipopolysaccharide induction of murine interleukin-1 beta. DNA Cell. Biol. 13:561.
Lischwe et al. (1993) Escherichia coli: derived murine interleukin-1 beta with N-terminus partially N alpha-acetylated. Protein Expr. Purif. 4:499.
Newton et al. (1993) An ELISA assay for murine interleukin-1 beta. J. Immunol. Methods. 161:257.
van Oostrum et al. (1991) The structure of murine interleukin-1 beta at 2.8 A resolution. J. Struct. Biol. 107:189.